Reply To: procedure for clotting/clumping during infusion of HPCs

  • Mary Lynn Rae

    October 5, 2014 at 10:27 pm

    We gravity drip our cells with a 170-260 micron filter set. We put a stopcock on the end with an empty 10 mL syringe if the cells get stuck.    


    Mary Lynn Rae
    Ann & Robert H. Lurie Children’s Hospital of Chicago
    Chicago, IL
    United States

    Original Message:
    Sent: 10-02-2014 12:41 PM
    From: Tobi Reisig
    Subject: procedure for clotting/clumping during infusion of HPCs

    Have other centers had issues with clotting or clumping of the cells during infusion of fresh or cryopreserved PBSCs or cord infusions.  For several years our procedure has been to closely monitor the clottin/clumping in the line and when it is at the “y” we disconnect the reaction line and aspirate the clots/clumps with a syringe.  This does  not happen very often but we do occasionally see this.  I have been looking at replacing this procedure with a 170 micron filter that can be added to the end of the line to catch any clotting/clumping of cells in the line.  The filter is made by ICU medical.  Wondering if others have seen this or had this issue and what they do?  Anyone else add a 170 or larger filter.  If clotting/clumping is detected in the bag before spiking we add a 170-260 micron blood filter.


    Tobi Reisig
    Alberta Children’s Hospital
    Calgary, AB